Digital PCR (dPCR) uses the procedure of end-point PCR but splits the PCR reaction into many single partitions, in which the template is randomly distributed across all available partitions. After PCR, the amplification target is detected by measuring the fluorescence – of either sequence-specific DNA probes or intercalating dyes – in all positive partitions. As the template is distributed randomly, Poisson statistics can be used to calculate the average amount of target DNA per valid, analyzable partition. The total amount of target DNA in all partitions of a well is calculated by multiplying the amount of average target DNA per partition with the number of valid partitions. Calculation of target concentration is determined by referring to the volume in all analyzable partitions, that is, partitions which were filled with reaction mix. The total number of filled partitions is identified by a fluorescent dye present in the reaction mix itself. Absolute quantification by dPCR eliminates the need for standard curves to determine amounts of target DNA in a given sample.

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Digital Droplet PCR

Rajarata University of Sri Lanka


Product Category/ Test Name (Matrix):

Digital Droplet PCR

Sub Category:

QIAcuity® Digital PCR System

Keywords:

PCR, DNA amplification, microdroplet technology, droplet intelligence, Fully automatic



Service Charge (Rs) -









Manufacturer Germany Funding Source ADB Project
Model QIAcuity One 2plex 911000 Whether the equipment service can be provided to external researchers/institutions Yes
Manufactured Year 2021 Availability of Technically Competent Staff
HS Code Instrument Condition Functioning well
Vendor Name Microtech Biological (Pvt) Ltd Commencement of Operations Date 2021-04-30
Vendor Contact 0115742693 InstrumentPrice 8400000.00
Vendor Url http://microtech-bio.com Stage of the Record Incomplete
Catalog Aceess
Catalog Upload View File
Catalog Link
Institute Rajarata University of Sri Lanka
Faculty Technology
Department Bioprocesses Technology
Laboratory Bioprocess Technology Laboratory
Contact Person Name Dr. T. C. Bamunuarachchige
Email heroiraj@gmail.com
Phone Number 0252266382
Mobile Number 0718096400
Product Category Digital Droplet PCR
Sub Category QIAcuity® Digital PCR System
Testing Paremeters Absolute quantification, Mutation detection, Genome editing, Copy number variation, Gene expression
Description The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in under 2 hours.
Relevant Instruments
Technical Specification
Number of samples per cycle 0
Number of samples per day 0
Total usage hour per day 0


Under Development Stage

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