PDS-1000/He™ System
The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.
The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells
Key Features
PDS-1000/He System with Hepta™ Adaptor
The Hepta adaptor for the PDS-1000/He biolistic system enables 7–10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000/He system where it splits the helium shock wave over seven macrocarriers. By uniformly spreading the DNA-coated gold or tungsten particles over this larger area, the system maximizes the number of cells transformed during one bombardment. As the helium is split seven ways, pressure and particle velocities are reduced, making the system ideal for plants and cell cultures requiring less forceful penetration.
Manufacturer | Biorad | Funding Source | Asian Development Bank |
Model | PDS-1000 | He™ and Hepta™ Systems | Whether the equipment service can be provided to external researchers/institutions | Yes |
Manufactured Year | 0000 | Availability of Technically Competent Staff | Yes |
HS Code | Instrument Condition | Partly malfunctioning | |
Vendor Name | Commencement of Operations Date | ||
Vendor Contact | InstrumentPrice | 0.00 | |
Vendor Url | Stage of the Record | Completed | |
Catalog Aceess | https://www.bio-rad.com/en-lk/product/pds-1000-he-hepta-systems?ID=1730e08d-f43a-46ea-b7f3-7b35c04c36eb | ||
Catalog Upload | View File | ||
Catalog Link | https://www.bio-rad.com/en-lk/product/pds-1000-he-hepta-systems?ID=1730e08d-f43a-46ea-b7f3-7b35c04c36eb |
Institute | University of Peradeniya |
Faculty | Agriculture |
Department | Agricultural Biotechnology Centre |
Laboratory | Agricultural Biotechnology Centre |
Contact Person Name | Prof. Pradeepa Bandaranayake |
agbc@agri.pdn.ac.lk | |
Phone Number | 0812387180 |
Mobile Number |
Product Category | Molecular Biology and Biotechnology |
Sub Category | |
Testing Paremeters | PCR amplification, Melt Curve analysis |
Description | |
Relevant Instruments |
Technical Specification | SpecificationsConstruction Aluminum, ABS plastic, and acrylic chassis Weight 15 kg Electrical input voltage 100–120 VAC, 50–60 Hz Maximum current <5 A
Mechanical
Fuse 6.3 A, 250 V, 5 x 20 mm Vacuum <0.4 inches mercury/min leakage Overpressure 0.5 psi relief valve, self-resetting
Environmental
Operating Temperature: 0–35°C (32–95°F) Storage Temperature: 0–70°C (32–158°F) |
Number of samples per cycle | 0 |
Number of samples per day | 0 |
Total usage hour per day | 0 |