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Functioning well

RT-qPCR

University of Peradeniya
  •   Faculty Agriculture
  •   Department Animal Science
  •   Laboratory Research laboratory
  •   Category Real Time PCR Machine
  •   Sub Category Rotor Gene Q 5 plex
Service Charge Contact Institute
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Manufactured Year 0000
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Instrument Condition Functioning well
Instrument Price (Rs) 0.00
Funding Source EU grant
External Research Support Yes
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Commencement of Operations 0000-00-00
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Stage of the Record Completed
Testing Service Real Time PCR Machine
Sub Category Rotor Gene Q 5 plex
Testing Parameters Up to 6 separate channels (365–680 nm excitation, 460–750 nm detection), Fixed optical path, separate high-power excitation LEDs and emission filters per channel, Highly sensitive photomultiplier (PMT) detector with gain setting (sensitivity control), Dynamic range: 10 orders of magnitude (assay dependent), PCR Tubes 0.2 ml: 0.2 ml x 36 wells, 15–50 µl recommended reaction volume, individual tubes with caps
Description Quantitative, real-time PCR is a precision science that places high demands on the instrument, chemistry, and software. Highthermal and optical uniformity, short equilibration times, and fast ramping rates are critical for precise and rapid quantitativeanalysis. Sensitivity, speed, and specificity are also highly dependent on the performance of the DNA polymerase andreaction components.
Relevant Instruments —
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Number of Samples per Cycle 0
Number of Samples per Day 0
Total Usage Hours per Day 0
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