| Testing Paremeters |
Up to 6 separate channels (365–680 nm excitation, 460–750 nm detection), Fixed optical path, separate high-power excitation LEDs and emission filters per channel, Highly sensitive photomultiplier (PMT) detector with gain setting (sensitivity control), Dynamic range: 10 orders of magnitude (assay dependent), PCR Tubes 0.2 ml: 0.2 ml x 36 wells, 15–50 µl recommended reaction volume, individual tubes with caps |
| Description |
Quantitative, real-time PCR is a precision science that places high demands on the instrument, chemistry, and software. Highthermal and optical uniformity, short equilibration times, and fast ramping rates are critical for precise and rapid quantitativeanalysis. Sensitivity, speed, and specificity are also highly dependent on the performance of the DNA polymerase andreaction components. |