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About This Instrument

The Illumina NextSeq 550 is a flexible, mid-throughput next-generation sequencer designed for a wide range of genomic applications. It uses two-channel Sequencing by Synthesis chemistry to generate high-quality short-read data with read lengths up to 2 × 150 bp. The system supports whole-exome sequencing, RNA-seq, targeted panels, and small-genome sequencing. With interchangeable Mid-Output and High-Output flow cells, it can produce 16–120 Gb per run, making it suitable for medium-scale projects. A key advantage is its built-in array scanning capability, enabling genotyping and methylation analysis. Overall, the NextSeq 550 provides reliable performance, flexibility, and efficient workflows.

Functioning well

NextSeq 550

Sri Lanka Institute of Biotechnology
  •   Department Molecular Diagnostics and Vaccine
  •   Laboratory Instrumentation Core Facility
  •   Category Next-Generation Sequencing
  •   Sub Category Whole-genome sequencing, Whole-exome sequencing
  •   Keywords Illumina NextSeq 550, NextSeq 550 system, Illumina NGS, Next-generation sequencing, Benchtop sequencer, Sequencing by synthesis, High-output sequencing, Rapid-run mode, Flow cell technology, Paired-end sequencing, High data output, Whole genome sequencing, Whole exome sequencing, Targeted sequencing, RNA sequencing, Transcriptomics, Gene expression profiling, Small RNA sequencing, Metagenomics, Microbiome analysis, Clinical research sequencing, Oncology sequencing
Service Charge Contact Institute
Manufacturer Illumina
Model Miseq
Manufactured Year 0000
HS Code —
Instrument Condition Functioning well
Instrument Price (Rs) 0.00
Funding Source —
External Research Support Yes
Availability of Technical Staff Yes
Commencement of Operations —
Vendor Name —
Vendor Contact —
Vendor URL —
Catalog Access —
Catalog Upload —
Catalog Link https://www.illumina.com/systems/sequencing-platforms/nextseq.html
Stage of the Record Completed
Testing Service Next-Generation Sequencing
Sub Category Whole-genome sequencing, Whole-exome sequencing
Testing Parameters —
Description Whole Exome Sequencing and Whole Genome Sequencing for a Maximum Coverage of 30x (Human, Animal, Plant and Microbial) Genome Assembly
Relevant Instruments Miseq, NextSeq 550
Technical Specification <p>System Specifications</p> <ul> <li> <p>Output range</p> 16.25 - 120 Gb</li> <li> <p>Reads per run</p> 130 - 400 million</li> <li> <p>Max read length</p> 2 &times; 150 bp</li> <li> <p>Run time</p> 12 -30 hr</li> </ul>
Number of Samples per Cycle 0
Number of Samples per Day 0
Total Usage Hours per Day 0
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