The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring six decoupled channels and white LED) and featuring six independent Veriflex temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

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Real-Time PCR Machine

Sri Lanka Institute of Biotechnology


Product Category/ Test Name (Matrix):

Disease Diagnostic Services

Sub Category:

PCR Based Molecular Diagnostic Services, Neuromuscular Disorder Diagnostics Services, Triplet Repeat Disorders Diagnostics Services, Infectious Disease Diagnostics Services

Keywords:





Service Charge (Rs) -









Manufacturer Applied Biosystems Funding Source
Model QuantStudio 5 Whether the equipment service can be provided to external researchers/institutions Yes
Manufactured Year 0000 Availability of Technically Competent Staff Yes
HS Code Instrument Condition Functioning well
Vendor Name Commencement of Operations Date
Vendor Contact InstrumentPrice 0.00
Vendor Url Stage of the Record Completed
Catalog Aceess
Catalog Upload View File
Catalog Link https://www.thermofisher.com/order/catalog/product/A28574
Institute Sri Lanka Institute of Biotechnology
Faculty
Department Molecular Diagnostics and Vaccine
Laboratory PCR Facility
Contact Person Name Dr Tharangika Bowange
Email tharangika.bowange@slibtec.gov.lk
Phone Number 0713028463
Mobile Number 0713028463
Product Category Disease Diagnostic Services
Sub Category PCR Based Molecular Diagnostic Services, Neuromuscular Disorder Diagnostics Services, Triplet Repeat Disorders Diagnostics Services, Infectious Disease Diagnostics Services
Testing Paremeters
Description

1. PCR Based Molecular Diagnostic Services

2. Neuromuscular Disorder Diagnostics Services

  • Molecular Diagnosis of  Duchenne Muscular Dystrophy and Becker Muscular Dystrophy by MLPA.
  • Molecular Diagnosis of Spinal Muscular Atrophy by Deletion Analysis of Survival Motor Neuron (SMN1) Gene by MLPA.

3. Triplet Repeat Disorders Diagnostics Services

  • Molecular diagnosis of Huntington disease
  • Molecular diagnosis of Spino Cerebellar Ataxias (SCA1, SCA2, SCA3, SCA6, SCA12)

4. Infectious Disease Diagnostics Services

  • Leptospirosis
  • Dengue
  • Chikungunya
  • Human Papilloma Virus
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Technical Specification

Optical & Detection

  • Excitation source: Bright-white LED light source for fluorescence excitation.

  • Optical detection: CMOS camera with 6 decoupled filters for multi-color detection (up to 6 targets multiplexing).

  • Excitation/Detection ranges: ~450–680 nm (excitation) / ~500–730 nm (detection).

Thermal & Cycling

  • Temperature range: ~4 °C to 99.9 °C.

  • Heating/cooling method: Peltier thermal block (precise active control).

  • Maximum block ramp rate: Up to ~6.5 °C/sec.

  • Average sample ramp rate: ~3.66 °C/sec.

  • Thermal uniformity: ~0.5 °C across wells.

  • Thermal accuracy: ~0.25 °C.

Reaction & Capacity

  • Block formats: Common configurations include 96-well (0.1 mL or 0.2 mL plate); some variant kits offer 384-well formats too.

  • Valid reaction volume: Typically 10–100 µL (for 0.2 mL blocks).

  • Dynamic range: Roughly 10 logarithmic units.

  • Sensitivity: Detects down to ~1 copy of target and can resolve ~1.5-fold changes in singleplex reactions.

Multiplexing & Dyes

  • Multiplex capability: Up to 5-plex with a passive reference or 6-plex without a passive reference.

  • Pre-calibrated dye compatibility: Includes common fluorescent dyes such as FAM™, SYBR™, VIC™, ABY™, NED™, TAMRA™, JUN™, ROX™, Mustang Purple™, and Cy®5.

Number of samples per cycle 0
Number of samples per day 0
Total usage hour per day 0


Under Development Stage

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