About This Instrument

The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring six decoupled channels and white LED) and featuring six independent Veriflex temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

Functioning well

Real-Time PCR Machine

Sri Lanka Institute of Biotechnology

Department Molecular Diagnostics and Vaccine
Laboratory PCR Facility
Category Disease Diagnostic Services
Sub Category PCR Based Molecular Diagnostic Services, Neuromuscular Disorder Diagnostics Services, Triplet Repeat Disorders Diagnostics Services, Infectious Disease Diagnostics Services

Service Charge Contact Institute

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Manufacturer	Applied Biosystems
Model	QuantStudio 5
Manufactured Year	0000
HS Code	—
Instrument Condition	Functioning well
Instrument Price (Rs)	0.00
Funding Source	—
External Research Support	Yes
Availability of Technical Staff	Yes
Commencement of Operations	—
Vendor Name	—
Vendor Contact	—
Vendor URL	—
Catalog Access	—
Catalog Upload	—
Catalog Link	https://www.thermofisher.com/order/catalog/product/A28574
Stage of the Record	Completed

Testing Service	Disease Diagnostic Services
Sub Category	PCR Based Molecular Diagnostic Services, Neuromuscular Disorder Diagnostics Services, Triplet Repeat Disorders Diagnostics Services, Infectious Disease Diagnostics Services
Testing Parameters	—
Description	1. PCR Based Molecular Diagnostic Services 2. Neuromuscular Disorder Diagnostics Services Molecular Diagnosis of  Duchenne Muscular Dystrophy and Becker Muscular Dystrophy by MLPA. Molecular Diagnosis of Spinal Muscular Atrophy by Deletion Analysis of Survival Motor Neuron (SMN1) Gene by MLPA. 3. Triplet Repeat Disorders Diagnostics Services Molecular diagnosis of Huntington disease Molecular diagnosis of Spino Cerebellar Ataxias (SCA1, SCA2, SCA3, SCA6, SCA12) 4. Infectious Disease Diagnostics Services Leptospirosis Dengue Chikungunya Human Papilloma Virus
Relevant Instruments	—

Technical Specification	<p><strong>Optical & Detection</strong></p> <ul> <li> <p><strong>Excitation source:</strong> Bright-white LED light source for fluorescence excitation.</p> </li> <li> <p><strong>Optical detection:</strong> CMOS camera with <strong>6 decoupled filters</strong> for multi-color detection (up to 6 targets multiplexing).</p> </li> <li> <p><strong>Excitation/Detection ranges:</strong> ~450–680 nm (excitation) / ~500–730 nm (detection).</p> </li> </ul> <p><strong>Thermal & Cycling</strong></p> <ul> <li> <p><strong>Temperature range:</strong> ~4 °C to 99.9 °C.</p> </li> <li> <p><strong>Heating/cooling method:</strong> Peltier thermal block (precise active control).</p> </li> <li> <p><strong>Maximum block ramp rate:</strong> Up to ~6.5 °C/sec.</p> </li> <li> <p><strong>Average sample ramp rate:</strong> ~3.66 °C/sec.</p> </li> <li> <p><strong>Thermal uniformity:</strong> ~0.5 °C across wells.</p> </li> <li> <p><strong>Thermal accuracy:</strong> ~0.25 °C.</p> </li> </ul> <p><strong>Reaction & Capacity</strong></p> <ul> <li> <p><strong>Block formats:</strong> Common configurations include 96-well (0.1 mL or 0.2 mL plate); some variant kits offer 384-well formats too.</p> </li> <li> <p><strong>Valid reaction volume:</strong> Typically <strong>10–100 µL</strong> (for 0.2 mL blocks).</p> </li> <li> <p><strong>Dynamic range:</strong> Roughly <strong>10 logarithmic units</strong>.</p> </li> <li> <p><strong>Sensitivity:</strong> Detects down to <strong>~1 copy</strong> of target and can resolve ~1.5-fold changes in singleplex reactions.</p> </li> </ul> <p><strong>Multiplexing & Dyes</strong></p> <ul> <li> <p><strong>Multiplex capability:</strong> Up to <strong>5-plex with a passive reference</strong> or <strong>6-plex without a passive reference</strong>.</p> </li> <li> <p><strong>Pre-calibrated dye compatibility:</strong> Includes common fluorescent dyes such as FAM™, SYBR™, VIC™, ABY™, NED™, TAMRA™, JUN™, ROX™, Mustang Purple™, and Cy®5.</p> </li> </ul>
Number of Samples per Cycle	0
Number of Samples per Day	0
Total Usage Hours per Day	0

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About This Instrument

Real-Time PCR Machine

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