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About This Instrument

The Cytek Northern Lights is a full-spectrum flow cytometer designed to offer high flexibility, sensitivity, and affordable multicolor analysis. Using spectral detection and advanced unmixing algorithms, it captures the complete emission profile of each fluorochrome, enabling efficient resolution of overlapping dyes and expanded panel design. It provides high-quality data for immunophenotyping, functional assays, and complex multicolor experiments while maintaining ease of use. With its compact design and cost-effective performance, the Northern Lights is well suited for research labs seeking versatile spectral cytometry.

Functioning well

Cytek Northern Lights

Sri Lanka Institute of Biotechnology
  •   Department Molecular Diagnostics and Vaccine
  •   Laboratory Immunology Laboratory
  •   Category Flowcytometry
  •   Sub Category Flowcytometry Analysis, Enzyme Linked Immunosorbent Assay (ELISA), Rapid Antigen Test (RAT) Development, Multimode Plate Reader Analysis, SDS-PAGE & Western Blots
  •   Keywords Cytek Northern Lights, Northern Lights flow cytometer, Full-spectrum flow cytometry, Spectral cytometry, Cytek Biosciences, Advanced optical design, APD detectors, Spectral unmixing, High-sensitivity detection, Wide dynamic range, Multicolor flow cytometry, Immunology research, Cell subset characterization, Rare event detection, Clinical research, Stem cell analysis, Cancer immunophenotyping, Functional assays, Cytokine profiling, Apoptosis assays, Cell cycle analysis
Service Charge Contact Institute
Manufacturer Cytek
Model Northern Lights
Manufactured Year 0000
HS Code —
Instrument Condition Functioning well
Instrument Price (Rs) 0.00
Funding Source —
External Research Support Yes
Availability of Technical Staff Yes
Commencement of Operations —
Vendor Name —
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Stage of the Record Completed
Testing Service Flowcytometry
Sub Category Flowcytometry Analysis, Enzyme Linked Immunosorbent Assay (ELISA), Rapid Antigen Test (RAT) Development, Multimode Plate Reader Analysis, SDS-PAGE & Western Blots
Testing Parameters —
Description Flowcytometry Analysis Enzyme Linked Immunosorbent Assay (ELISA) Rapid Antigen Test (RAT) Development  Multimode Plate Reader Analysis SDS-PAGE & Western Blots
Relevant Instruments Cytek Northern lights
Technical Specification <h3>Key Technical Specifications</h3> <ul> <li> <p><strong>Laser configurations:</strong> 1&ndash;3 lasers</p> <ul> <li> <p>3-laser: 405 nm (100 mW), 488 nm (50 mW), 640 nm (80 mW)</p> </li> </ul> </li> <li> <p><strong>Detection capability / Colors:</strong></p> <ul> <li> <p>Up to <strong>24+ colors</strong> with 3-laser system</p> </li> </ul> </li> <li> <p><strong>Detectors:</strong></p> <ul> <li> <p>Utilizes proprietary high-sensitivity semiconductor arrays (CWDM) per laser for spectral detection across a broad emission range (&asymp; 420&ndash;829 nm) &mdash; no filter swapping required when changing fluorochromes.</p> </li> <li> <p>Module breakdown (for three-laser config): &ldquo;Violet&rdquo; module &mdash; 16 channels; &ldquo;Blue&rdquo; module &mdash; 14 channels; &ldquo;Red&rdquo; module &mdash; 8 channels.</p> </li> </ul> </li> <li> <p><strong>Fluidics &amp; Throughput:</strong></p> <ul> <li> <p>Sample flow rates: low 15 &mu;L/min, medium 30 &mu;L/min, high 60 &mu;L/min; optional plate-based high-throughput mode up to 100 &mu;L/min.</p> </li> <li> <p>Supports manual tubes (12 &times; 75 mm) and optionally 96-well plates (with plate loader accessory).</p> </li> <li> <p>Data acquisition rate: up to ~ 35,000 events per second (three-laser system).</p> </li> <li> <p>Carryover (tube mode) typically &lt; 0.1%.</p> </li> </ul> </li> <li> <p><strong>Optics &amp; Scatter Detection:</strong></p> <ul> <li> <p>Forward Scatter (FSC): high-performance semiconductor detector with 488 nm bandpass filter.</p> </li> <li> <p>Side Scatter (SSC): detectors available for 405 nm and 488 nm side scatter (405 nm SSC only in systems with the violet laser).</p> </li> <li> <p>Sensitivity: capable of resolving small particles (e.g. 0.2 &micro;m beads from noise).</p> </li> </ul> </li> <li> <p><strong>Optical &amp; Spectral Performance:</strong></p> <ul> <li> <p>Fixed optical assembly &mdash; no need to change optical filters when changing fluorochromes.</p> </li> <li> <p>Full-spectrum detection + unmixing for fluorochromes with overlapping emission spectra (e.g. APC vs Alexa 647) &mdash; enables flexible design of multicolor panels.</p> </li> </ul> </li> </ul>
Number of Samples per Cycle 0
Number of Samples per Day 0
Total Usage Hours per Day 0
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