The BD FACSCanto II Flow Cytometer is a multicolor clinical flow cytometry system developed by BD Biosciences for rapid analysis of blood and cellular samples. It uses multiple lasers and fluorescence detectors to identify and quantify different cell populations based on surface antigen expression. The instrument is widely used in hematology, immunology, oncology, and stem cell transplantation laboratories because of its high sensitivity, automation, and reproducibility.
In CD34+ enumeration, the BD FACSCanto II plays an important role in identifying and counting hematopoietic stem and progenitor cells expressing the CD34 antigen. CD34+ cell counting is essential for monitoring stem cell mobilization, assessing stem cell harvest quality, and determining suitability for hematopoietic stem cell transplantation. The system commonly uses the modified ISHAGE protocol together with BD Stem Cell Enumeration reagents to provide accurate absolute CD34+ counts and percentages.
| Manufacturer | Becton Dickinson & Company, USA | Funding Source | AHEAD Grant |
| Model | BD FACSCanto II | Whether the equipment service can be provided to external researchers/institutions | Yes |
| Manufactured Year | 2019 | Availability of Technically Competent Staff | Yes |
| HS Code | Instrument Condition | Functioning well | |
| Vendor Name | A Baur & Co. (Pvt.) Ltd. | Commencement of Operations Date | 2019-09-26 |
| Vendor Contact | 0114728779 | InstrumentPrice | 15990000.00 |
| Vendor Url | https://www.baurs.com/ | Stage of the Record | Completed |
| Catalog Aceess | |||
| Catalog Upload | View File | ||
| Catalog Link | https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD_FACSCanto_II_brochure.pdf |
| Institute | University of Colombo |
| Faculty | Faculty of Medicine |
| Department | Department of Pathology |
| Laboratory | Haematology |
| Contact Person Name | Professor Lallindra Gooneratne |
| lallindra@path.cmb.ac.lk | |
| Phone Number | |
| Mobile Number | 0773667687 |
| Product Category | 1. Biological Fluids (Plasma/Serum) |
| Sub Category | |
| Testing Paremeters | 1. Biological Samples Blood (Plasma/Serum).Ex; Lipid Profile, Liver Profile, Serum Electrolytes. |
| Description | |
| Relevant Instruments | Name - BA-88AModel - Mindray Semi Automated Analyzer Serial Number - WR-43013994 |
| Technical Specification | https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/facscanto_techspecs.pdf BD FACSCanto II Flow Cytometer — Key Features General Description Benchtop flow cytometer designed for clinical and research laboratories Supports: 2-laser configurations 3-laser / 8-color configurations Detects up to: 8 fluorescence colors plus FSC and SSC scatter parameters Optimized for: automation reproducibility multicolor flow cytometry reduced technician hands-on time Optical System Standard Lasers 488 nm blue solid-state laser — 20 mW 633 nm red HeNe laser — 17 mW Optional Third Laser 405 nm violet diode laser — 30 mW Flow Cell Quartz rectangular flow cell: 180 × 430 μm Resolution FSC resolution: 1.0 μm SSC resolution: 0.5 μm Detector Configuration Standard 6 PMTs (4-2 configuration) Optional 8 PMTs: 4-2-2 configuration (3-laser) 5-3 configuration (2-laser) Supported Fluorochromes Blue Laser FITC PE PerCP / PerCP-Cy5.5 PE-Cy7 Red Laser APC APC-Cy7 Alexa Fluor 700 (optional configuration) Violet Laser Pacific Blue AmCyan Sensitivity Threshold Sensitivity FITC: <100 MESF PE: <50 MESF Acquisition Speed Up to 10,000 events/second with 6 compensated fluorescence parameters + 2 scatter parameters Fluidics Flow Rates Minimum: 10 μL/min Maximum: 120 μL/min Carryover ≤0.1% Sample Dead Volume 30 μL Automated Maintenance Automated: startup shutdown cleaning cycles Data & Software Signal Processing 18-bit dynamic range IEEE 32-bit floating-point resolution Parameters Recorded Area (A) Width (W) Height (H) Time (T) Software BD FACSDiva v6.1.3 BD FACSCanto Clinical v2.2 / v2.4 Operating System Microsoft Windows XP Pro Optional Accessories BD FACS Loader 40-tube carousel Barcode support Automated sample loading BD High Throughput Sampler Supports: 96-well plates 384-well plates Throughput: <15 min per 96-well plate Physical Specifications Dimensions Cytometer 24 × 36 × 25 in (61 × 91 × 64 cm) Fluidics Cart 24 × 31 × 25 in (61 × 79 × 64 cm) Weight Cytometer: 145 kg Fluidics cart: 51 kg Power 100/115/230 VAC 50–60 Hz |
| Number of samples per cycle | 1 |
| Number of samples per day | 0 |
| Total usage hour per day | 0 |
